HEPES, phosphate, etc) without detrimental effects. F-actin can be transferred to a variety of buffers (e.g. F-actin requires ATP (0.2 mM) and Mg2+ (2 mM) for stability and is unstable below pH 6.5 and above pH 8.5. F-actin is stable and can be stored at 4☌ for 1-2 weeks. Question 2: What is the best way to store F-actin after polymerizing?Īnswer 2: G-actin is stable for two days at 4☌ and requires a divalent cation, pH 6.5 - 8.0 and ATP for stability. When thawing frozen aliquots, it is important to thaw in a room temperature water bath. The frozen aliquots will be stable for 6 months. Alternatively, actins can be immediately resuspended at the concentration recommended, aliquoted, snap-frozen in liquid nitrogen and stored at -70☌. With proper storage, the lyophilized actins are guaranteed to be stable for 6 months from the date of purchase. It is important to monitor the freshness of the desiccant and insure that it continues to absorb moisture to protect the lyophilized actins. Upon receipt, the lyophilized powders should be stored at 4☌ in a sealed container with desiccant. Question 1: What is the best way to store actin proteins to insure maximum stability and shelf-life?Īnswer 1: Cytoskeleton provides all of our actin proteins as lyophilized powders so that they can be shipped at room temperature. Cooperation between actin-binding proteins of invasive Salmonella: SipA potentiates SipC nucleation and bundling of actin. The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro.
An actin nucleation mechanism mediated by Bni1 and profilin. Direct regulation of Arp2/3 complex activity and function by the actin binding protein coronin. Probing polymerization forces by using actin-propelled lipid vesicles. Espin cross-links cause the elongation of microvillus-type parallel actin bundles in vivo. Coordinated regulation of actin filament turnover by a high-molecular-weight Srv2/CAP complex, cofilin, profilin, and Aip1. Subdomain organization of the Acanthamoeba myosin IC tail from cryo-electron microscopy. Gelsolin mediates collagen phagocytosis through a rac-dependent step. Expression of actin-interacting protein 1 suppresses impaired chemotaxis of Dictyostelium cells lacking the Na-H exchanger NHE1. Functional role of inositol-1,4,5-trisphosphate-3-kinase-A for motility of malignant transformed cells. Growth-Arrest-Specific Protein 2 Inhibits Cell Division in Xenopus Embryos. Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoforms. Effects of Basic Calponin on the Flexural Mechanics and Stability of F-Actin. Aβ mediates F-actin disassembly in dendritic spines leading to cognitive deficits in alzheimer’s disease. Archives of biochemistry and biophysics vol. Cleavage of loops 1 and 2 in skeletal muscle heavy meromyosin (HMM) leads to a decreased function.
Transgelin 2 is required for embryo implantation by promoting actin polymerization. Characterizing the actin-binding ability of Zasp52 and its contribution to myofibril assembly. Triggering Cation-Induced Contraction of Cytoskeleton Networks via Microfluidics. Actin and microtubule crosslinkers tune mobility and control co-localization in a composite cytoskeletal network. The load dependence and the force-velocity relation in intact myosin filaments from skeletal and smooth muscles. Hsiao BY, et al., Human Costars Family Protein ABRACL Modulates Actin Dynamics and Cell Migration and Associates with Tumorigenic Growth Int. Phase separation-mediated condensation of Whirlin-Myo15-Eps8 stereocilia tip complex ( 2021)
Al, Secreted gelsolin inhibits DNGR-1-dependent cross-presentation and cancer immunity ( 2021) Al, Myosin-driven actin-microtubule networks exhibit self-organized contractile dynamics DOI: 10.1126/sciadv.abe4334 ( 2021) UBE2元, a Partner of MuRF1/TRIM63, Is Involved in the Degradation of Myofibrillar Actin and Myosin. MemGlow™ Plasma Membrane Probes for Bioimaging 8.Small G-protein Activators & Inhibitors 5.GOBlot - The First Affordable Western Blot Processor.New Signal-Seeker™: PTM detection kits, antibodies, and reagents.